Factors Affecting Clonal Multiplication in Kokam (Garcinia Indica Choicy.)

Abstract

In vitro studies in Kokarn (Garcinia indica) were carried to standardize protocol for aseptic culture shoot establishment of Kokam. During the investigation, treatments for surface sterilization, explant size, stage of explants and gelling agents were studied. Studies revealed that the HgC12 at 0. 1 % for 4 minutes and HgC1i at 0. 1 % concentration for 5 minutes were found to be at par as the best surface sterilization treatments to obtain maximum Kokam shoot establishment in vitro. The shoot tip explant of length 4 cm in length, dark green coloured were the ideal explants for in vitro establishment. Incorporation of Phytagel as a gelling agent in the Murashige and Skoog's medium exerted good conditions for in vitro shoot establishment in Kokarn.