Decolorization of Sulphonated Azodye Metanil Yellow By Newly Isolated Bacterial Strain- Bacillus -3330

Abstract

Newly isolated Bacillus sp. MTCC-3330 was screened for the decolorization of a sulphonated azodye metanil yellow under aerobic conditions. 100% decolorization was observed of Metanil Yellow at 0.01 mM, 0.02mM 0.05mM concentrations within 30h, 35h and 45h respectively. Decolorization was confirmed by UV-VIS spectrophotometer. The initial dye solution showed highest peak at the wavelength of 437 nm (A.max of the Metanil Yellow). The decolorized dye showed disappearance of peak, which indicated that the decolorization is due to dye degradation. The bacterium sp. also decolorized the another azodye Methyl Orange.